Journal: Bioactive Materials

Article Title: A composite hydrogel enables the spatiotemporal delivery of distinct cytokines to drive the native vascularized bone regeneration

doi: 10.1016/j.bioactmat.2026.02.048

Figure Lengend Snippet: Angiogenic capacity formulations of HUVECs in response to different composite biomaterial in vitro. A) Calcein/PI staining of HUVECs seeded on glass slides, showing the cell migration profiles of HUVECs treated with different material groups, scale bar = 200 μm; B) Quantitative analysis of the intercellular blank areas in each group, with the baseline group serving as the negative control; C) Angiogenic images of HUVECs co-cultured with different composite materials for 4 h and 8 h respectively, scale bar = 250 μm; D–G) Quantitative assessment of angiogenic capacity in each group via ImageJ software analysis of key angiogenic parameters. Abbreviations: NC = negative control group; V = exogenous VEGF protein-only group; GV=GelMA + exogenous VEGF protein group; GVE = GelMA + VEGF + ECM group; GVEP= GelMA/VEGF + ECM/PCSK9 group. Statistical notations: ∗∗means that compared with the control group, p < 0.01; ns = no significant difference between group.

Article Snippet: A Calcein/PI Cell Viability/Cytotoxicity Assay Kit (Beyotime, China) was used to recognize the living and dead cells.

Techniques: In Vitro, Staining, Migration, Negative Control, Cell Culture, Software, Control

Journal: Science Advances

Article Title: Biaxially ductile supramolecular polymer networks

doi: 10.1126/sciadv.aeb7157

Figure Lengend Snippet: ( A ) Fluorescence images obtained through live-dead staining of mouse chondrogenic cells (ATDC5) cocultured with the SPN extracts (left) and blank control (right) for 24 hours at 37.0°C, in which more images of the same cells are shown in fig. S34. ( B ) Photographs of a resistive biaxial strain sensor based upon a cross-shaped SPN specimen. Plot of relative resistance change in the X and Y axis for the SPN versus time obtained through ( C ) applying incremental equal biaxial strains from 17 to 100% and ( D ) applying orthogonal biaxial trains, in which γ ( X ) = 17% and γ ( Y ) = 0% and then γ ( X ) = 0% and γ ( Y ) = 17%. ( E ) Schematic of a SPN patch on a lung model for real-time monitoring of biaxial surface extension. ( F ) Plot of relative resistance change in the X and Y axis for the SPN versus time obtained through applying the same rate (24 BPM) yet incremental depths of respiration from 3, 6, to 9 liters min −1 on a lung model (inserted photographs). ( G ) Schematic of a SPN patch attached to three different positions on a lung model for real-time monitoring of biaxial surface extension. ( H ) Plot of relative resistance change in the X and Y axis for the SPN at position 1 (left, side-upper), position 2 (middle, side-lower), and position 3 (right, front-upper) obtained through applying the same rate and depth of respiration on a lung model (inserted photographs). SPN was prepared at C M = 2.0 M with 2.5 mol % BPyVI-NVI-CB[8].

Article Snippet: After the cells were cocultured with the extract for 24 hours, cells were stained using a Calcein/PI Live/Dead Viability/Cytotoxicity Assay Kit (Beyotime Biotechnology, C2015S) for 20 min. After washing twice with phosphate-buffered saline, the cells were imaged under a fluorescence microscope.

Techniques: Fluorescence, Staining, Control